PRCC Polyclonal antibody

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  WB analysis of HeLa using 12019-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12019-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PRCC Polyclonal Antibody for WB, ELISA

Cat No. 12019-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PRCC, Proline rich protein PRCC, RCCP1, TPRC

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12019-1-AP targets PRCC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2638

  Product name: Recombinant human PRCC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 173-491 aa of BC010450

  Sequence: GSSEGTGLSALLPQPKNLTVKETNRLLLPHAFSRKPSDGSPDTKPSRLASKTKTSSLAPVVGTTTTTPSPSAIKAAAKSAALQVTKQITQEEDDSDEEVAPENFFSLPEKAEPPGVEPYPYPIPTVPEELPPGTEPEPAFQDDAANAPLEFKMAAGSSGAPWMPKPGDDYSYNQFSTYGDANAAGAYYQDYYSGGYYPAQDPALVPTQEIAPDASFIDDEAFKRLQGKRNRGREEINFVEIKGDDQLSGAQQWMTKSLTEEKTMKSFSKKKGEQPTGQQRRKHQITYLIHQAKERELELKNTWSENKLSRRQTQAKYGF

• Full Name: papillary renal cell carcinoma (translocation-associated)
• Calculated Molecular Weight: 491 aa, 52 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC010450
• Gene Symbol: PRCC
• Gene ID (NCBI): 5546
• RRID: AB_2169667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92733
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRCC, also named as, TPRC,regulate cell cycle progression through interaction with MAD2L2. It is frequently found in papillary renal cell carcinoma. Endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA plicing factors including SC35, PRL1 and CDC5.