PRCP Polyclonal antibody

PRCP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15995-1-AP

Synonyms

Angiotensinase C, HUMPCP, Lysosomal carboxypeptidase C, PCP, PRCP, Proline carboxypeptidase, Prolylcarboxypeptidase

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Validation Data Gallery

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  WB analysis using 15995-1-AP

  A549 lysates were subjected to SDS PAGE followed by western blot with 15995-1-AP (PRCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 15995-1-AP

  IP result of anti-PRCP (IP:15995-1-AP, 4ug; Detection:15995-1-AP 1:300) with L02 cells lysate 1800ug.

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  IHC staining of human lung cancer using 15995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15995-1-AP (PRCP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 15995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15995-1-AP (PRCP Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

15995-1-AP targets PRCP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRCP fusion protein Ag8869
• Full Name: prolylcarboxypeptidase (angiotensinase C)
• Calculated Molecular Weight: 496 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC001500
• Gene Symbol: PRCP
• Gene ID (NCBI): 5547
• RRID: AB_2169673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRCP(Lysosomal Pro-X carboxypeptidase) is also named as PCP and belongs to the peptidase S28 family. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. PRCP is both a PK activator and an angiotensin II inactivator indicates another previously unappreciated interaction between the plasma kallikrein/kinin and the renin-angiotensin systems(PMID:11830581).Some previous gelfiltration and dynamic-light scattering results suggested that PRCP is a dimer in solution(PMID:20540760). The full length protein has a signal peptide, a propeptide and six glycosylation sites and can exsit as 65 kDa modified protein.

Publications

Species	Application	Title
mouse	WB,IF	Acta Pharmacol Sin; α-MSH-catabolic enzyme prolylcarboxypeptidase in nucleus accumbens shell ameliorates stress susceptibility in mice through regulating synaptic plasticity; Authors - Qiao Deng
human	WB	Carcinogenesis; Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.; Authors - Christelle Lenain
human	WB	Dose Response; Decrease of Prolylcarboxypeptidase Dose of Aqueous Humor is Involved in the Pathogenesis of Primary Open-Angle Glaucoma via Finetuning of the Local Ocular Renin-Angiotensin System; Authors - Jing Ren