PRDM15 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRDM15 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25590-1-AP

Synonyms

C21orf83, PFM15, PR domain containing 15, PRDM15, Zinc finger protein 298, ZNF298

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Validation Data Gallery

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  WB analysis of Jurkat cells using 25590-1-AP

  WB result of PRDM15 (25590-1-AP, 1:500) with si-control and si-PRDM15 transfected Jurkat cells.

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  WB analysis of MCF-7 using 25590-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25590-1-AP (PRDM15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 25590-1-AP

  IP result of anti-PRDM15 (IP:25590-1-AP, 5ug; Detection:25590-1-AP 1:500) with MCF-7 cells lysate 2500ug.

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Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 25590-1-AP is PRDM15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRDM15 fusion protein Ag22296
• Full Name: PR domain containing 15
• Calculated Molecular Weight: 1507 aa, 169 kDa
• Observed Molecular Weight: 169 kDa
• GenBank Accession Number: BC067102
• Gene Symbol: PRDM15
• Gene ID (NCBI): 63977
• RRID: AB_2880145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; The RNA methyltransferase METTL16 enhances cholangiocarcinoma growth through PRDM15-mediated FGFR4 expression; Authors - Nianli Liu ; KD Validated