PREP Polyclonal antibody

• Featured Product
• KD/KO Validated

PREP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11536-1-AP

Synonyms

Post-proline cleaving enzyme, PEP, EC:3.4.21.26

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Validation Data Gallery

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  WB analysis of human lung using 11536-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11536-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11536-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11536-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11536-1-AP (PREP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 11536-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human lung tissue, Jurkat cells, mouse lung tissue
• Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

11536-1-AP targets PREP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PREP fusion protein Ag2101
• Full Name: prolyl endopeptidase
• Calculated Molecular Weight: 710 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC030636
• Gene Symbol: PREP
• Gene ID (NCBI): 5550
• RRID: AB_2284249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)

Publications

Species	Application	Title
human	IHC	Gut; Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease.; Authors - Koelink Pim J PJ
human	WB,IHC,IF	Oxid Med Cell Longev; Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration.; Authors - Huo-Liang Zheng ; KD Validated
human	WB	Int J Biol Macromol; A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.; Authors - Hui Zeng
human	WB,IHC,IF	PLoS One; Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation?; Authors - Overbeek Saskia A SA
mouse	IHC	Am J Physiol Lung Cell Mol Physiol; Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown.; Authors - Braber Saskia S
	IF	J Gen Virol; Phosphorylation of the Autographa californica multiple nucleopolyhedrovirus polyhedron envelope protein plays an important role in the formation of the occlusion body envelope.; Authors - Jia Wang