PREX1 Polyclonal antibody, PBS Only

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• PREX1 Polyclonal antibody, PBS Only (33193-1-PBS)

• 

  WB analysis using 33193-1-AP (same clone as 33193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33193-1-AP (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33193-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 33193-1-AP (same clone as 33193-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody (33193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33193-1-PBS in a different storage buffer formulation.

PREX1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 33193-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA1415, P REX1, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,4,5)-dependent Rac exchanger 1

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Product Information

33193-1-PBS targets PREX1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36649

  Product name: Recombinant human PREX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 700-900 aa of NM_020820.3

  Sequence: LLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVEN

• Full Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 186 kDa
• GenBank Accession Number: NM_020820.3
• Gene Symbol: PREX1
• Gene ID (NCBI): 57580
• RRID: AB_3742888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TCU6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529).