PRICKLE1 Recombinant antibody

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  WB analysis of MDA-MB-231 using 84926-1-RR

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84926-1-RR (PRICKLE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 84926-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84926-1-RR (PRICKLE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84926-1-RR against Human PRICKLE1 were performed. The affinity constant is 7.50 nM.

PRICKLE1 Recombinant Antibody for WB, IHC, ELISA

Cat No. 84926-1-RR

Clone No.242506A8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

242506A8, EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84926-1-RR targets PRICKLE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRICKLE1 fusion protein Ag18407
• Full Name: prickle homolog 1 (Drosophila)
• Calculated Molecular Weight: 831 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC114939
• Gene Symbol: PRICKLE1
• Gene ID (NCBI): 144165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96MT3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).