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PRIM1 Polyclonal antibody, PBS Only (10773-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 10773-1-AP (same clone as 10773-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10773-1-AP (same clone as 10773-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of Sp2/0 using 10773-1-AP (same clone as 10773-1-PBS)
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 10773-1-AP (same clone as 10773-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of Sp2/0 using 10773-1-AP (same clone as 10773-1-PBS)
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 10773-1-AP (same clone as 10773-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10773-1-AP (same clone as 10773-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 10773-1-AP (same clone as 10773-1-PBS)
IP result of anti-PRIM1 (IP:10773-1-AP, 4ug; Detection:10773-1-AP 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 10773-1-AP (same clone as 10773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10773-1-AP (PRIM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 10773-1-AP (same clone as 10773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
IHC staining of human thyroid cancer using 10773-1-AP (same clone as 10773-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10773-1-AP (same clone as 10773-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRIM1 antibody (10773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10773-1-PBS in a different storage buffer formulation.

PRIM1 Polyclonal antibody, PBS Only

Name: PRIM1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10773-1-PBS

PRIM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10773-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10773-1-PBS targets PRIM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1124 Product name: Recombinant human PRIM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 121-420 aa of BC005266 Sequence: CCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF Predict reactive species
Full Name	primase, DNA, polypeptide 1 (49kDa)
Calculated Molecular Weight	50 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC005266
Gene Symbol	PRIM1
Gene ID (NCBI)	5557
RRID	AB_2237549
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49642
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex.