Tested Applications
| Positive WB detected in | human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55382-1-AP targets PRKACB in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase, cAMP-dependent, catalytic, beta |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 36-55 kDa |
| GenBank Accession Number | NM_002731 |
| Gene Symbol | PRKACB |
| Gene ID (NCBI) | 5567 |
| RRID | AB_10949078 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKACB antibody 55382-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PRKACB Polyclonal antibody (Cat# 55382-1-AP) has 43 citations published in journals including Neuron, Theranostics, Cell Death & Disease, Phytomedicine, Free Radical Biology and Medicine, Journal of Ethnopharmacology, International Journal of Molecular Sciences, and others. Research fields span neuroscience (depression, neurodegeneration, stroke), oncology (breast, gastric, renal, and glioblastoma cancers), liver metabolism, pharmacology, immunology, reproductive biology, and endocrinology.
| Species | Application | Title |
|---|---|---|
Theranostics The delta subunit of the GABAA receptor is necessary for the GPT2-promoted breast cancer metastasis | ||
Cell Death Dis Mesenchymal stem cells in tumor microenvironment: drivers of bladder cancer progression through mitochondrial dynamics and energy production | ||
Pharmacol Res Hypericin attenuates nonalcoholic fatty liver disease and abnormal lipid metabolism via the PKA-mediated AMPK signaling pathway in vitro and in vivo. | ||
Cell Death Dis Loss of PRKACB facilitates metastasis of diffuse-type gastric cancer through RhoA signaling activation.
| ||
Cell Commun Signal Olanzapine suppresses mPFC activity-norepinephrine releasing to alleviate CLOCK-enhanced cancer stemness under chronic stress |
