PKA C-beta-specific Polyclonal antibody

PKA C-beta-specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55382-1-AP

Synonyms

PKA C beta, PKACB, PRKACB



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

55382-1-AP targets PKA C-beta-specific in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein kinase, cAMP-dependent, catalytic, beta
Calculated molecular weight 46 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberNM_002731
Gene symbol PRKACB
Gene ID (NCBI) 5567
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. This antibody is specific to PRKACB.

Protocols

Product Specific Protocols
WB protocol for PKA C-beta-specific antibody 55382-1-APDownload protocol
IHC protocol for PKA C-beta-specific antibody 55382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Ethnopharmacol

Total barley maiya alkaloids inhibit prolactin secretion by acting on dopamine D2 receptor and protein kinase A targets.

Authors - Xiaoyun Gong
humanWB

Proteomics

Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.

Authors - Hwee Tong Tan
WB

Phytomedicine

Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics.

Authors - Yan-Ling Qiao
humanWB

Pharmacol Res

Hypericin attenuates nonalcoholic fatty liver disease and abnormal lipid metabolism via the PKA-mediated AMPK signaling pathway in vitro and in vivo.

Authors - Chen Liang