PRKAR2A Monoclonal antibody, PBS Only

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  WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

PRKAR2A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67751-1-PBS

Clone No.1B2B3

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PKR2, PRKAR2, PRKAR2A

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Product Information

67751-1-PBS targets PRKAR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29893

  Product name: Recombinant human PRKAR2A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 74-382 aa of BC002763

  Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

• Full Name: protein kinase, cAMP-dependent, regulatory, type II, alpha
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002763
• Gene Symbol: PRKAR2A
• Gene ID (NCBI): 5576
• RRID: AB_2918520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13861
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.