Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | mouse liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, U-251 cells, HeLa cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19132-1-AP targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5631 Product name: Recombinant human PRKCD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 326-676 aa of BC043350 Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED Predict reactive species |
| Full Name | protein kinase C, delta |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC043350 |
| Gene Symbol | PKC Delta |
| Gene ID (NCBI) | 5580 |
| RRID | AB_10637263 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PKC Delta antibody 19132-1-AP | Download protocol |
| IF protocol for PKC Delta antibody 19132-1-AP | Download protocol |
| IHC protocol for PKC Delta antibody 19132-1-AP | Download protocol |
| IP protocol for PKC Delta antibody 19132-1-AP | Download protocol |
| WB protocol for PKC Delta antibody 19132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PKCδ (19132-1-AP) has 9 total citations published in journals including Phytomedicine, Cell Death Discovery, Oncogene, Journal of Ethnopharmacology, Neurochemistry International, Virology Sinica, iScience, Fish & Shellfish Immunology, and Food and Chemical Toxicology. Associated research fields span pulmonary fibrosis and pyroptosis, diabetic wound healing, gastric cancer metastasis, neuroinflammation, Zika virus infection, gut inflammation, crustacean immunology, and pancreatic β-cell dysfunction.
| Species | Application | Title |
|---|---|---|
Phytomedicine Rottlerin inhibits PKCδ to attenuate pulmonary fibrosis by suppressing NLRC4/ASC-mediated pyroptosis | ||
Cell Death Discov Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing | ||
Oncogene TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD | ||
J Ethnopharmacol Tetrastigma hemsleyanum suppresses neuroinflammation in febrile seizures rats via regulating PKC-δ/caspase-1 signaling pathway | ||
Neurochem Int Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes. | ||
Virol Sin Rottlerin plays an antiviral role at early and late steps of Zika virus infection
|
Reviews
What customers say
Based on available reviews, this antibody received a 5-star rating from a user who tested it at a 1:2000 dilution on mouse brain lysate for Western Blot. The reviewer confirmed it detected a band at the expected molecular weight, describing it as "a good antibody for WB with the right MW." Overall, the feedback is positive, with no reported issues regarding specificity or performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
B (Verified Customer) (07-11-2022) | A good antibody for WB with the right MW.
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