• Featured Product
  • KD/KO Validated

PKC Iota Polyclonal antibody

PKC Iota Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 13883-1-AP

Synonyms

aPKC lambda/iota, DXS1179E, PKC iota, PKCI, PRKC lambda/iota, PRKCI, Protein kinase C iota type, protein kinase C, iota



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13883-1-AP targets PKC Iota in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity chicken, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Iota fusion protein Ag4990
Full Name protein kinase C, iota
Calculated molecular weight 68 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC022016
Gene symbol PRKCI
Gene ID (NCBI) 5584
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Iota antibody 13883-1-APDownload protocol
IHC protocol for PKC Iota antibody 13883-1-APDownload protocol
IF protocol for PKC Iota antibody 13883-1-APDownload protocol
IP protocol for PKC Iota antibody 13883-1-APDownload protocol
FC protocol for PKC Iota antibody 13883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIHC,IF

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
  • KD Validated
humanWB,IHC

Onco Targets Ther

Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.

Authors - Liu Liu
  • KD Validated
humanWB,IF

Mol Cancer

circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.

Authors - Wei Gao
  • KD Validated