PRKCQ-Specific Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PRKCQ-Specific Polyclonal antibody, PBS Only (19982-1-PBS)

• 

  WB analysis using 19982-1-AP (same clone as 19982-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19982-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19982-1-AP (same clone as 19982-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19982-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 19982-1-AP (same clone as 19982-1-PBS)

  IP result of anti-PRKCQ-Specific (IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with K-562 cells lysate 1600 ug. This data was developed using the same antibody clone with 19982-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 19982-1-AP (same clone as 19982-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug PRKCQ-Specific Polyclonal antibody (19982-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19982-1-PBS in a different storage buffer formulation.

PRKCQ-Specific Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA

Cat No. 19982-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

PRKCQ, EC:2.7.11.13, nPKC-theta, PRKCT, protein kinase C

---

Product Information

19982-1-PBS targets PRKCQ-Specific in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase C, theta
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 72-74 kDa
• GenBank Accession Number: NM_006257
• Gene Symbol: PRKCQ
• Gene ID (NCBI): 5588
• RRID: AB_3669319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04759
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.