PRKCSH Recombinant monoclonal antibody

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• PRKCSH Recombinant monoclonal antibody (87004-3-RR)

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  WB analysis using 87004-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87004-3-RR (PRKCSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 87004-3-RR

  Biolayer interferometry (BLl) kinetic assays of 87004-3-RR against Human PRKCSH were performed. The affinity constant is 6.09 nM.

PRKCSH Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87004-3-RR

Clone No.252090C1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

80K H protein, 80K-H protein, G19P1, Glucosidase 2 subunit beta, Glucosidase II subunit beta

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HEK-293T cells, Daudi cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87004-3-RR targets PRKCSH in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2796

  Product name: Recombinant human PRKCSH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 157-506 aa of BC013586

  Sequence: TVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPTPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL

• Full Name: protein kinase C substrate 80K-H
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC013586
• Gene Symbol: PRKCSH
• Gene ID (NCBI): 5589
• RRID: AB_3745267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14314
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.