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  • KD/KO Validated

PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10860-1-AP

Synonyms

PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10860-1-AP targets PKC Zeta in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Zeta fusion protein Ag1310
Full Name protein kinase C, zeta
Calculated molecular weight 80 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC014270
Gene symbol PRKCZ
Gene ID (NCBI) 5590
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Zeta antibody 10860-1-APDownload protocol
IHC protocol for PKC Zeta antibody 10860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1.

Authors - Alejandro M Chibly
  • KO Validated