EPCR/CD201 Monoclonal antibody, PBS Only

• 

  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67658-1-Ig (same clone as 67658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67658-1-Ig (same clone as 67658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 67658-1-Ig (same clone as 67658-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67658-1-PBS in a different storage buffer formulation.

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 67658-1-PBS

Clone No.1F5C4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

---

Product Information

67658-1-PBS targets EPCR/CD201 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25628

  Product name: Recombinant human PROCR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 18-210 aa of BC014451

  Sequence: SQDASDGLQRLHMLQISYFRDPYHVWYQGNASLGGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLVHQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALWQADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTKGSQTSRSYTS

• Full Name: protein C receptor, endothelial (EPCR)
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014451
• Gene Symbol: PROCR
• Gene ID (NCBI): 10544
• RRID: AB_2882855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UNN8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.