PROCR Monoclonal antibody

PROCR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F5C4

Cat no : 67658-1-Ig

Synonyms

Activated protein C receptor, APC receptor, bA42O4.2, CCCA, CCD41, CD201, Endothelial protein C receptor, EPCR, PROCR

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Validation Data Gallery

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  WB analysis using 67658-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67658-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 67658-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67658-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67658-1-Ig targets PROCR in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PROCR fusion protein Ag25628
• Full Name: protein C receptor, endothelial (EPCR)
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014451
• Gene Symbol: PROCR
• Gene ID (NCBI): 10544
• RRID: AB_2882855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Signal Transduct Target Ther; Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.; Authors - Panpan Zhang