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PROS1 Polyclonal antibody, PBS Only (16910-1-PBS)

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
WB analysis using 16910-1-AP (same clone as 16910-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 16910-1-AP (same clone as 16910-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
WB analysis of mouse placenta using 16910-1-AP (same clone as 16910-1-PBS)
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 16910-1-AP (PROS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 16910-1-AP (same clone as 16910-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16910-1-AP (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (16910-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 16910-1-AP (same clone as 16910-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.
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1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PROS1 (16910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16910-1-PBS in a different storage buffer formulation.

PROS1 Polyclonal antibody, PBS Only

Name: PROS1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16910-1-PBS

PROS1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 16910-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PROS, Vitamin K-dependent protein S

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

16910-1-PBS targets PROS1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10539 Product name: Recombinant human PROS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 328-676 aa of BC015801 Sequence: DFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS Predict reactive species
Full Name	protein S (alpha)
Calculated Molecular Weight	75 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC015801
Gene Symbol	PROS1
Gene ID (NCBI)	5627
RRID	AB_2300207
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07225
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.