Tested Applications
| Positive WB detected in | DU 145 cells, human placenta tissue, mouse placenta tissue, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16910-1-AP targets PROS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, plasmodium falciparum |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10539 Product name: Recombinant human PROS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 328-676 aa of BC015801 Sequence: DFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS Predict reactive species |
| Full Name | protein S (alpha) |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC015801 |
| Gene Symbol | PROS1 |
| Gene ID (NCBI) | 5627 |
| RRID | AB_2300207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07225 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PROS1 antibody 16910-1-AP | Download protocol |
| IF protocol for PROS1 antibody 16910-1-AP | Download protocol |
| IHC protocol for PROS1 antibody 16910-1-AP | Download protocol |
| WB protocol for PROS1 antibody 16910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PROS1 (Protein S) antibody (Cat# 16910-1-AP) has 20 citations published in journals including Science, Nature Methods, Nature Communications, Brain, Cell Reports, and Scientific Reports. Research fields span immunology, oncology (breast, melanoma, bladder cancer, meningioma), hematology (protein S deficiency, anticoagulation), neuroscience (stroke, white matter disease), cardiology, nephrology, and infectious disease (malaria, schizophrenia). Applications include WB, IF, IHC, and ELISA in human and mouse models.
| Species | Application | Title |
|---|---|---|
Science The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity. | ||
Nat Methods Screening cell-cell communication in spatial transcriptomics via collective optimal transport | ||
Nat Commun A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution | ||
Cell Commun Signal Targeting TYRO3 attenuates apoptotic cell-driven cancer stemness in triple negative breast cancer. | ||
Reviews
What customers say
The product received a 5-star rating from a researcher who used it for Western Blot on liver tissue at a 1:1000 dilution. The reviewer reported a clean, strong band, indicating reliable performance and specificity for their application. Overall, the feedback highlights the antibody's effectiveness in WB with a simple, positive experience.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hongxue (Verified Customer) (01-25-2021) | Nice band for WB
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