PRPF3 Polyclonal antibody, PBS Only

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• PRPF3 Polyclonal antibody, PBS Only (10106-1-PBS)

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  WB analysis of Y79 using 10106-1-AP (same clone as 10106-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10106-1-AP (same clone as 10106-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 10106-1-AP (same clone as 10106-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10106-1-AP (same clone as 10106-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10106-1-AP (PRPF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 10106-1-AP (same clone as 10106-1-PBS)

  IP result of anti-PRPF3 (IP:10106-1-AP, 5ug; Detection:10106-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 10106-1-AP (same clone as 10106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10106-1-AP (PRPF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10106-1-PBS in a different storage buffer formulation.

PRPF3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10106-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

RP18, Prp3p, PRP3, Pre-mRNA-splicing factor 3, HPRP3P

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Product Information

10106-1-PBS targets PRPF3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0154

  Product name: Recombinant human PRPF3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 229-493 aa of BC000184

  Sequence: MVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQDP

• Full Name: PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 90 kDa, 78 kDa
• GenBank Accession Number: BC000184
• Gene Symbol: PRPF3
• Gene ID (NCBI): 9129
• RRID: AB_2170994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43395
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)