PRPF31 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  WB analysis using 86315-1-RR (same clone as 86315-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86315-1-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86315-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02586-1

  The mean PRPF31 concentration was determined to be 14.26 ng/mL in HeLa cell extract based on a 1.10 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02586-1

  Sandwich ELISA standard curve of MP02586-1, Human PRPF31 Recombinant Matched Antibody Pair - PBS only. 86315-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26716. 86315-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

PRPF31 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 86315-1-PBS

Clone No.251005G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

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Product Information

86315-1-PBS targets PRPF31 as part of a matched antibody pair:

MP02586-1: 86315-1-PBS capture and 86315-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26716

  Product name: Recombinant human PRPF31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 101-212 aa of NM_015629

  Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

• Full Name: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_015629
• Gene Symbol: PRPF31
• Gene ID (NCBI): 26121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWY3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.