PRPF31 Recombinant monoclonal antibody

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  WB analysis using 86315-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86315-2-RR (PRPF31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86315-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 86315-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF31 antibody (86315-2-RR, Clone: 251005H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 86315-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86315-2-RR against Human PRPF31 were performed. The affinity constant is below 1 pM.

PRPF31 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86315-2-RR

Clone No.251005H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

hPrp31, NY BR 99, Protein 61K, PRP31, RP11

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, A549 cells, Caco-2 cells, OVCAR-3 cells
• Positive IF/ICC detected in: Jurkat cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86315-2-RR targets PRPF31 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26716

  Product name: Recombinant human PRPF31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 101-212 aa of NM_015629

  Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

• Full Name: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_015629
• Gene Symbol: PRPF31
• Gene ID (NCBI): 26121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWY3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.