PRPF38A Polyclonal antibody

PRPF38A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24946-1-AP

Synonyms

RP5 965L7.1, Pre-mRNA-splicing factor 38A, Pre mRNA splicing factor 38A

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Validation Data Gallery

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  WB analysis of K-562 using 24946-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24946-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 24946-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24946-1-AP (PRPF38A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24946-1-AP targets PRPF38A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF38A fusion protein Ag20980
• Full Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
• Calculated Molecular Weight: 312 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC000777
• Gene Symbol: PRPF38A
• Gene ID (NCBI): 84950
• RRID: AB_2879814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF38A was identified as an essential splicing factor in yeast and was found to be important for the progression of the splicing process to the first splicing step in this organism. PRPF38A is one of nine splicing factors that are specifically incorporated during the formation of the pre-catalytic spliceosomal B complex (B-specific proteins) and that are released again during spliceosome activation3 (PMID: 35869234). PRPF38A knockdown led to widespread intronic retention and altered splicing of transcripts of genes implicated in protein metabolism, mitosis, proteasome function and apoptosis (PMID: 28878028).