PRPS1 Polyclonal antibody

PRPS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15549-1-AP

Synonyms

PRS I, PPRibP, KIAA0967, EC:2.7.6.1, CMTX5

---

Validation Data Gallery

• 

  WB analysis using 15549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15549-1-AP (PRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 15549-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15549-1-AP (PRPS1 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15549-1-AP

  IP result of anti-PRPS1 (IP:15549-1-AP, 4ug; Detection:15549-1-AP 1:100) with HeLa cells lysate 2600ug.

• 

  IHC staining of mouse testis using 15549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15549-1-AP (PRPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15549-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15549-1-AP (PRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 15549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PRPS1 antibody (15549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, rat spleen tissue, HeLa cells, HEK-293 cells, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15549-1-AP targets PRPS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPS1 fusion protein Ag7907
• Full Name: phosphoribosyl pyrophosphate synthetase 1
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001605
• Gene Symbol: PRPS1
• Gene ID (NCBI): 5631
• RRID: AB_10694269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPS1, also named as Ribose-phosphate pyrophosphokinase 1, is a 318 amino acid protein, which belongs to the ribose-phosphate pyrophosphokinase family. PRPS1 can form Homodimer and the active form is probably a hexamer composed of 3 homodimers. PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. PRPS1 as a module has an association with the Hepatocellular carcinoma.

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	CoIP,WB	Sci Adv; NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.; Authors - Shashank Srivastava
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Amino Acids; Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.; Authors - Chen Bai