PRPS2 Recombinant antibody

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  WB analysis using 85090-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85090-5-RR (PRPS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 85090-5-RR

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 85090-5-RR (PRPS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PRPS2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85090-5-RR

Clone No.242657G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242657G2, EC:2.7.6.1, PPRibP, PRS II, PRSII

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Tested Applications

• Positive WB detected in: Jurkat cells, rat spleen tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85090-5-RR targets PRPS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRPS2 fusion protein Ag25785
• Full Name: phosphoribosyl pyrophosphate synthetase 2
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC119662
• Gene Symbol: PRPS2
• Gene ID (NCBI): 5634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPS (phosphoribosyl pyrophosphate synthetase) proteins catalyze the synthesis of phosphoribosyl pyrophosphate (PRPP). Three human PRPS isoforms exist and are encoded by three different genes. PRPS1 and PRPS2 (also known as PRS1 and PRS2, respectively) are ubiquitously expressed, while PRPS3 (also known as PRPS1L1) is specific to the testis. PRPP is an important substrate synthesized from MgATP and ribose-5-phosphate in a reaction that requires inorganic phosphate and magnesium as a cofactor. PRPP is essential in the synthesis of nearly all nucleotides, implying that PRPS proteins play an important role in nucleotide biosynthesis and purine metabolism. PRPS2 is a 318 amino acid protein that exists as a homodimer and a hexamer composed of three homodimers.