Tested Applications
| Positive WB detected in | BxPC-3 cells, mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17814-1-AP targets PRPSAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12132 Product name: Recombinant human PRPSAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-369 aa of BC106050 Sequence: MFCVTPPELETKMNITKGGLVLFSANSNSSCMELSKKIAERLGVEMGKVQVYQEPNRETRVQIQESVRGKDVFIIQTVSKDVNTTIMELLIMVYACKTSCAKSIIGVIPYFPYSKQCKMRKRGSIVSKLLASMMCKAGLTHLITMDLHQKEIQGFFNIPVDNLRASPFLLQYIQEEIPDYRNAVIVAKSPASAKRAQSFAERLRLGIAVIHGEAQDAESDLVDGRHSPPMVRSVAAIHPSLEIPMLIPKEKPPITVVGDVGGRIAIIVDDIIDDVDSFLAAAETLKERGAYKIFVMATHGLLSSDAPRRIEESAIDEVVVTNTIPHEVQKLQCPKIKTVDISMILSEAIRRIHNGESMSYLFRNIGLDD Predict reactive species |
| Full Name | phosphoribosyl pyrophosphate synthetase-associated protein 2 |
| Calculated Molecular Weight | 369 aa, 41 kDa |
| Observed Molecular Weight | 37-41 kDa |
| GenBank Accession Number | BC106050 |
| Gene Symbol | PRPSAP2 |
| Gene ID (NCBI) | 5636 |
| RRID | AB_10598314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60256 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRPSAP2 antibody 17814-1-AP | Download protocol |
| IHC protocol for PRPSAP2 antibody 17814-1-AP | Download protocol |
| WB protocol for PRPSAP2 antibody 17814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRPSAP2 Polyclonal antibody (Cat# 17814-1-AP) has 4 total citations. These appear in Nature Communications (1) and bioRxiv (3). The associated research fields include evolutionary biology of the mammalian PRPS enzyme complex (gene duplication, paralog specialisation, divergent evolution across species) and cancer biology (PRPS activity in redox homeostasis within Myc-driven lymphoma). All cited applications are Western blot, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Nat Commun The role of gene duplication and paralog specialisation in the evolution of the mammalian PRPS complex
| ||
bioRxiv Evolutionary origins and innovations sculpting the mammalian PRPS enzyme complex
| ||
