PRPSAP2 Polyclonal antibody

PRPSAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17814-1-AP

Synonyms

PAP41, PRPSAP2

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Validation Data Gallery

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  WB analysis using 17814-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17814-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 17814-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 17814-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of BxPC-3 using 17814-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PRPSAP2 antibody (17814-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse brain tissue, HeLa cells, rat brain tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17814-1-AP targets PRPSAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPSAP2 fusion protein Ag12132
• Full Name: phosphoribosyl pyrophosphate synthetase-associated protein 2
• Calculated Molecular Weight: 369 aa, 41 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC106050
• Gene Symbol: PRPSAP2
• Gene ID (NCBI): 5636
• RRID: AB_10598314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.