PRR11 Polyclonal antibody

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  WB analysis using 27422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27422-1-AP (PRR11 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

PRR11 Polyclonal Antibody for WB, ELISA

Cat No. 27422-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Proline rich protein 11, proline rich 11

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, L02 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27422-1-AP targets PRR11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26636

  Product name: Recombinant human PRR11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 181-360 aa of BC008669

  Sequence: ASHFPPPPPPPPLPPPPPPLAPVLLRKPSLAKALQAGPLKKDGPMQITVKDLLTVKLKKTQSLDEKRKLIPSPKARNPLVTVSDLQHVTLKPNSKVLSTRVTNVLITPGKSQMDLRKLLRKVDVERSPGGTPLTNKENMETGTGLTPVMTQALRRKFQLAHPRSPTPTLPLSTSSFDEQN

• Full Name: proline rich 11
• Calculated Molecular Weight: 360 aa, 40 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC008669
• Gene Symbol: PRR11
• Gene ID (NCBI): 55771
• RRID: AB_3669600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HE9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRR11, or Proline-rich protein 11, is a protein-coding gene situated on chromosome 17q22-23, which plays a significant role in various cellular processes. The protein itself is composed of a nuclear localization signal (NLS), two proline-rich regions (PRs), and a zinc finger domain (ZFD) (PMID: 36551227). PRR11 has been implicated in the regulation of cell cycle, and it is involved in multiple aspects of cancer biology, including proliferation, migration, invasion, cell-cycle progression, apoptosis, autophagy, and chemotherapy resistance. It serves as a prognostic indicator in certain human cancers and is considered a potential target for therapeutic interventions (PMID: 36739300).