PRRG2 Polyclonal antibody

PRRG2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11618-1-AP

Synonyms

Transmembrane gamma-carboxyglutamic acid protein 2, TMG2, Proline-rich Gla protein 2, Proline-rich gamma-carboxyglutamic acid protein 2, PRGP2

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Validation Data Gallery

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  WB analysis using 11618-1-AP

  37°C incubated mouse kidney tissue and mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11618-1-AP (PRRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11618-1-AP targets PRRG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRRG2 fusion protein Ag2197
• Full Name: proline rich Gla (G-carboxyglutamic acid) 2
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 16-22 kDa
• GenBank Accession Number: BC026032
• Gene Symbol: PRRG2
• Gene ID (NCBI): 5639
• RRID: AB_2171684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline-rich Gla protein 2 (PRRG2) represents one of the four known transmembrane-carboxyglutamic acid proteins in vertebrates. As a founding member of the vitamin K-dependent, single-pass integral membrane protein family, PRRG2 is characterized by an extracellular N-terminus consisting of approximately 45 amino acids rich in Gla, as well as a conserved Pro/Leu-Pro-xaa-Tyr (PY) motif proximal to its C-terminus. PRRG2 primarily localizes to the cell surface, where the Gla domain is exposed extracellularly, serving as a pivotal gene node with positive autoregulatory function that plays a critical role in maintaining human hemostasis (PMID: 38227081). The calculated molecular weight of PRRG2 is usually approximately 22 kda.