Tested Applications
| Positive WB detected in | Raji cells, HepG2 cells, mouse thymus tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15005-1-AP targets Trypsin 2/PRSS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6461 Product name: Recombinant human PRSS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-239 aa of BC030260 Sequence: MNLLLILTFVAAAVAAPFDDDDKIVGGYICEENSVPYQVSLNSGYHFCGGSLISEQWVVSAGHCYKSRIQVRLGEHNIEVLEGNEQFINAAKIIRHPKYNSRTLDNDILLIKLSSPAVINSRVSAISLPTAPPAAGTESLISGWGNTLSSGADYPDELQCLDAPVLSQAECEASYPGKITNNMFCVGFLEGGKDSCQGDSGGPVVSNGELQGIVSWGYGCAQKNRPGVYTKVYNYVDWI Predict reactive species |
| Full Name | protease, serine, 2 (trypsin 2) |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa, 35 kDa |
| GenBank Accession Number | BC030260 |
| Gene Symbol | PRSS2 |
| Gene ID (NCBI) | 5645 |
| RRID | AB_2171841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07478 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The human pancreas produces three isoforms of trypsinogen encoded by separate genes, the PRSS1 (protease, serine 1), PRSS2, and PRSS3 genes. PRSS2 is also named as TRY2(trypsin-2), TRYP2. In the ileum, it may be involved in defensin processing, including DEFA5. The preproprotein comprises 247 amino acids, including a 15-amino acid signal peptide and an 8-amino acid activation peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Trypsin 2/PRSS2 antibody 15005-1-AP | Download protocol |
| WB protocol for Trypsin 2/PRSS2 antibody 15005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Trypsin 2/PRSS2 Polyclonal antibody (Cat# 15005-1-AP) has 5 citations published in Tissue Cell, Acta Histochem, Biochemical and Biophysical Research Communications, Biology Open, and Open Medicine. The research fields span gastric cancer prognosis and metastasis, pancreatic cancer epigenomics, exocrine pancreas protease biology in zebrafish and murine models, and papillary thyroid carcinoma immune correlates. Applications cited include Western blot and immunohistochemistry in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Tissue Cell PRSS2 overexpression relates to poor prognosis and promotes proliferation, migration and invasion in gastric cancer
| ||
Acta Histochem PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer
| ||
Biochem Biophys Res Commun Epigenome-wide association analysis of pancreatic exocrine cells from high-fat- and normal diet-fed mice and its potential use for understanding the oncogenesis of human pancreatic cancer | ||
Open Med (Wars) Prognostic relevance of PRSS2 and its immune correlates in papillary thyroid carcinoma. | ||
Biol Open Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems. |
