PRSS27 Polyclonal antibody

PRSS27 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19515-1-AP

Synonyms

CAPH2, Marapsin, MPN, Pancreasin, protease, serine 27, PRSS27, Serine protease 27

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Validation Data Gallery

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  WB analysis of SW 1990 using 19515-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 19515-1-AP (PRSS27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 19515-1-AP

  IP result of anti-PRSS27 (IP:19515-1-AP, 4ug; Detection:19515-1-AP 1:300) with SW 1990 cells lysate 2960ug.

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  IHC staining of human pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 19515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SW 1990 cells
• Positive IP detected in: SW 1990 cells
• Positive IHC detected in: human pancreas tissue, human oesophagus cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19515-1-AP targets PRSS27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protease, serine 27
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_031948
• Gene Symbol: PRSS27
• Gene ID (NCBI): 83886
• RRID: AB_2878585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRSS27, also named as CAPH2, MPN, belongs to the peptidase S1 family. The trypsin-like serine protease marapsin (Prss27) is a member of the large protease gene cluster at human chromosome 16p13.3. The Prss27 gene is expressed by a variety of human, mouse, and opossum tissues. In humans and mice, it is especially abundant in stratified squamous epithelia of cornea, larynx, esophagus, and cervix and is induced in wounded and psoriatic skin (PMID: 23447538). Prss27 expression was restricted to tissues containing stratified squamous epithelia and was absent or only weakly expressed in all other tissues, including the pancreas.