PRSS8 Polyclonal antibody

PRSS8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15527-1-AP

Synonyms

Prostasin light chain, Prostasin heavy chain, EC:3.4.21.-, Channel-activating protease 1, CAP1

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Validation Data Gallery

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  WB analysis of human colon using 15527-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15527-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15527-1-AP (PRSS8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15527-1-AP (PRSS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 15527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15527-1-AP (PRSS8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human colon tissue, mouse kidney tissue
• Positive IHC detected in: mouse ovary tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 5 publications below

Product Information

15527-1-AP targets PRSS8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRSS8 fusion protein Ag7234
• Full Name: protease, serine, 8
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001462
• Gene Symbol: PRSS8
• Gene ID (NCBI): 5652
• RRID: AB_2172168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protease serine S1 family member 8 (PRSS8), a membrane-anchored serine protease, has been reported to be involved in the development of several human cancers. PRSS8 protein level was highly expressed in well differentiated cancer cell and was lower or absent in poorly differentiated cancer cell. Significantly higher levels of prostasin were found in early stage OVC serum samples compared to benign ovarian and normal donor samples. Overexpression of PRSS8 mRNA and high levels of prostasin in multiple subtypes of early stage ovarian tumors may provide clinical biomarkers for early detection of OVC. (PMID: 27036110, PMID: 27050145)

Publications

Species	Application	Title
mouse	WB,IHC	Acta Physiol (Oxf); Zymogen-locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice.; Authors - Daniel Essigke
mouse	WB	Br J Pharmacol; PMID: 31222723; Authors - Ming-Ming Wu
human	WB,IHC	J Hypertens; Prostasin and matriptase (ST14) in placenta from preeclamptic and healthy pregnant women.; Authors - Britta Frederiksen-Møller
human	WB,IHC	Acta Physiol (Oxf); Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium.; Authors - Steensgaard M M
human	WB	Pflugers Arch; The epithelial Na+ channel α- and γ-subunits are cleaved at predicted furin-cleavage sites, glycosylated and membrane associated in human kidney.; Authors - Rikke Zachar
human	WB	Nephrol Dial Transplant; PMID: 25609736; Authors - Henrik Andersen