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  • KD/KO Validated

PRUNE2 Polyclonal antibody

PRUNE2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11458-1-AP

Synonyms

A214N16.3, BMCC1, BNIPXL, C9orf65, KIAA0367, Protein prune homolog 2, prune homolog 2 (Drosophila), PRUNE2, RP11 58J3.2



Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11458-1-AP targets PRUNE2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRUNE2 fusion protein Ag2025
Full Name prune homolog 2 (Drosophila)
Calculated Molecular Weight 341 kDa
Observed Molecular Weight 140~150 kDa
GenBank Accession NumberBC019095
Gene Symbol PRUNE2
Gene ID (NCBI) 158471
RRIDAB_2172439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRUNE2 gene, also named BMCC1, encodes protein prune homolog 2. PRUNE2 gene are overlapping with PCA3, one of the most prostate cancer specific markers. PRUNE2 may play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. Besides its high level of expression seen in the nervous system (brain, cerebellum and spinal cord), it is also expressed at high levels in noneuroblastoma, rhabdomyosarcoma, melanoma and some osteosarcoma cell lines, whereas at only low levels in cancer cell lines of liver, breast, thyroid and colon. The Calculated molecular weight of this protein is 340 kDa. Our antibody specifically recognises the 140-150 kDa band which is an isoform or cleaved fragment of PRUNE2 (PMID:21234814).

Protocols

Product Specific Protocols
IHC protocol for PRUNE2 antibody 11458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF, IP

Proc Natl Acad Sci U S A

PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3.

Authors - Ahmad Salameh
  • KD Validated
humanWB, IHC

Cell Death Dis

BMCC1, which is an interacting partner of BCL2, attenuates AKT activity, accompanied by apoptosis.

Authors - Y Tatsumi
humanWB,IHC

Exp Ther Med

PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo.

Authors - Ting Li
  • KD Validated
human,mouseIHC,WB

Technol Cancer Res Treat

FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer

Authors - Ting Li