PRDX3 Recombinant monoclonal antibody

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  WB analysis using 81833-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 81833-1-RR

  WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

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  IHC staining of human lung cancer using 81833-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 81833-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 81833-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 81833-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81833-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81833-1-RR against Human PRDX3 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

PRDX3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 81833-1-RR

Clone No.1L11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1L11, Antioxidant protein 1, AOP1, AOP-1, EC:1.11.1.24

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, A549 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81833-1-RR targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1062

  Product name: Recombinant human PRX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC007062

  Sequence: MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC007062
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• RRID: AB_2935583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30048
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.