Mouse PSAP Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86043-3-RR (same clone as 86043-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86043-3-RR (Psap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86043-3-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 86043-3-RR (same clone as 86043-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using PSAP antibody (86043-3-RR, Clone: 250312B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86043-3-PBS in a different storage buffer formulation.

PSAP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86043-3-PBS

Clone No.250312B12

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Prosaposin, Saposin-A, Saposin-B, Saposin-B-Val, Saposin-C

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Product Information

86043-3-PBS targets PSAP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3155

  Product name: Recombinant Mouse PSAP/Prosaposin protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-557 aa of NM_011179.3

  Sequence: SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

• Full Name: prosaposin
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_011179.3
• Gene Symbol: Psap
• Gene ID (NCBI): 19156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: NP_035309.3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Prosaposin (PSAP) is also named as Sulfated glycoprotein 1 (SGP-1). PSAP is a secreted neurotrophic factor and a lysosomal protein serving as the precursor of saposins (A-D), which are cofactors for hydrolases of sphingolipids (PMID: 25130661, PMID: 31220974). PSAP modulates glycosphingolipid metabolism and variants have been linked to Parkinson's disease (PD) (PMID: 37726325). As a dual-function protein, PSAP is a lysosome-associated protein that participates in a variety of cellular processes. In the lysosome, PSAP is processed to activate enzymes that degrade lipids. PSAP proteins located extracellularly are involved in cancer progression, such as proliferation and tumor death suppression signaling (PMID: 39405903). Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D (PMID: 33242172). Both human and mouse prosaposin are 65-70 kDa proteins cleaved in the lysosomes into four 10-15 kDa proteins designated saposins A, B, C and D (PMID: 8573994). Sulfated glycoprotein-1 (prosaposin) exists in 2 forms: a 65 kDa form targeted to lysosomes and a post-translationally modified to the 70 kDa form secreted extracellularly (PMID: 8593668).