PSAP Recombinant monoclonal antibody

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  WB analysis using 86043-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86043-3-RR (Psap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 86043-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using PSAP antibody (86043-3-RR, Clone: 250312B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PSAP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86043-3-RR

Clone No.250312B12

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Prosaposin, Saposin-A, Saposin-B, Saposin-B-Val, Saposin-C

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, RAW 264.7 cells, C6 cells, mouse cerebellum tissue, rat testis tissue, mouse testis tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86043-3-RR targets PSAP in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3155

  Product name: Recombinant Mouse PSAP/Prosaposin protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-557 aa of NM_011179.3

  Sequence: SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

• Full Name: prosaposin
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_011179.3
• Gene Symbol: Psap
• Gene ID (NCBI): 19156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: NP_035309.3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prosaposin (PSAP) is also named as Sulfated glycoprotein 1 (SGP-1). PSAP is a secreted neurotrophic factor and a lysosomal protein serving as the precursor of saposins (A-D), which are cofactors for hydrolases of sphingolipids (PMID: 25130661, PMID: 31220974). PSAP modulates glycosphingolipid metabolism and variants have been linked to Parkinson's disease (PD) (PMID: 37726325). As a dual-function protein, PSAP is a lysosome-associated protein that participates in a variety of cellular processes. In the lysosome, PSAP is processed to activate enzymes that degrade lipids. PSAP proteins located extracellularly are involved in cancer progression, such as proliferation and tumor death suppression signaling (PMID: 39405903). Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D (PMID: 33242172). Both human and mouse prosaposin are 65-70 kDa proteins cleaved in the lysosomes into four 10-15 kDa proteins designated saposins A, B, C and D (PMID: 8573994). Sulfated glycoprotein-1 (prosaposin) exists in 2 forms: a 65 kDa form targeted to lysosomes and a post-translationally modified to the 70 kDa form secreted extracellularly (PMID: 8593668).