PSAPL1 Polyclonal antibody

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  WB analysis of multi-cells/tissue using 55128-1-AP

  WB result of 55128-1-AP.

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  WB analysis of human testis using 55128-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55128-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55128-1-AP (PSAPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 55128-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55128-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55128-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAPL1 antibody (55128-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

PSAPL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55128-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Saposin B-Val-like, Saposin B-like, Saposin A-like, PSAP L1, prosaposin like 1

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Tested Applications

• Positive WB detected in: human testis tissue, multi-cells/tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55128-1-AP targets PSAPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: prosaposin-like 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-63 kDa
• GenBank Accession Number: NM_001085382
• Gene Symbol: PSAPL1
• Gene ID (NCBI): 768239
• RRID: AB_10888638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6NUJ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSAPL1 is a protein like PSAP. It can be cleaved to be 5 chains: Saposin A-like, Saposin B-Val-like, Saposin B-like, Saposin C-like and Saposin D-like. PSAPL1 may activate the lysosomal degradation of sphingolipids. The antibody is specific to PSAPL1.