Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81106-1-RR targets PSD95-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | discs, large homolog 4 (Drosophila) |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 90-95 kDa |
| GenBank Accession Number | NM_001365 |
| Gene Symbol | PSD95 |
| Gene ID (NCBI) | 1742 |
| RRID | AB_2923703 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSD95-Specific antibody 81106-1-RR | Download protocol |
| IHC protocol for PSD95-Specific antibody 81106-1-RR | Download protocol |
| WB protocol for PSD95-Specific antibody 81106-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSD95-Specific Recombinant monoclonal antibody (Cat# 81106-1-RR) has 2 citations. One appears in Advanced Science (2026), studying 3D-bioprinted brain-like transplants for Alzheimer's disease-like dementia using IHC and IF in rat. The other is in CNS Neuroscience & Therapeutics (2025), investigating transcranial iTBS combined with trans-spinal iTBS targeting the PDE1A/cAMP/PKA axis for neural regeneration after spinal cord injury. The associated research fields include neurodegenerative disease, stem cell therapy, and spinal cord injury repair.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Advanced Stem Cell Therapy: 3D-Bioprinted Brain-Like Transplants for Alzheimer's Disease-Like Dementia. | ||
CNS Neurosci Ther Transcranial iTBS Combined With Trans-Spinal iTBS Targeting PDE1A/cAMP/PKA Axis Regulates Neural Regeneration After Spinal Cord Injury |
Reviews
What customers say
Both reviewers rated this recombinant antibody 5 stars for immunofluorescence. One reported "very beautiful staining." The other used it at a 1/1000 dilution on mouse brain slices and achieved good results, noting that as their first use of a recombinant antibody, its performance was entirely comparable to a traditional one. Overall, the reviews highlight strong staining quality and reliable performance in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sheeny (Verified Customer) (01-23-2026) | very beautiful staining
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Baptiste (Verified Customer) (05-19-2023) | Good results at 1/1000 dilution on mouse brain slices. This was our first use of a recombinant antibody and it is entirely comparable to a traditional one.
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