CoraLite® Plus 647-conjugated PSD95-Specific Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated PSD95-Specific Recombinant monoclonal antibody (CL647-81106)

PSD95-Specific Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL647-81106

Clone No.1I22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

DLG4, PSD-95, psd95/dlg4, DLG 4, PSD 95

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  IF Staining of mouse brain using CL647-81106

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 647 PSD95-Specific antibody (CL647-81106, Clone: 1I22 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-81106 targets PSD95-Specific in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: discs, large homolog 4 (Drosophila)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: NM_001365
• Gene Symbol: PSD95
• Gene ID (NCBI): 1742
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78352
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).