PSG11 Polyclonal antibody

• 

  WB analysis of mouse uterus using 16352-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16352-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16352-1-AP (PSG11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16352-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16352-1-AP (PSG11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16352-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSG11 antibody (16352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PSG11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16352-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Pregnancy-specific beta-1-glycoprotein 11, Pregnancy-specific beta-1-glycoprotein 13, Pregnancy-specific glycoprotein 11, Pregnancy-specific glycoprotein 13, PS beta G 11

---

Tested Applications

• Positive WB detected in: mouse uterus tissue, HeLa cells
• Positive IHC detected in: human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16352-1-AP targets PSG11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9562

  Product name: Recombinant human PSG11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-219 aa of BC020711

  Sequence: MHAAEIMGPLSAPPCTEHIKWKGLLLTVETPKPSISSSNLNPREAMETVILTCNPETPDASYLWWMNGQSLPMTHRMQLSETNRTLFLFGVTKYTAGPYECEIWNSGSASRSDPVTLNLLHGPDLPRIFPSVTSYYSGENLDLSCFADSNPPAQYSWTINGKFQLSGQKLFIPQITPKHNGLYACSARNSATGEESSTSLTIRVIAPPGLGTFAFNNPT

• Full Name: pregnancy specific beta-1-glycoprotein 11
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC020711
• Gene Symbol: PSG11
• Gene ID (NCBI): 5680
• RRID: AB_2171065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQ72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Front Genet; Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.; Authors - Christopher B Nelson