Tested Applications
| Positive WB detected in | mouse uterus tissue, HeLa cells |
| Positive IHC detected in | human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16352-1-AP targets PSG11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9562 Product name: Recombinant human PSG11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC020711 Sequence: MHAAEIMGPLSAPPCTEHIKWKGLLLTVETPKPSISSSNLNPREAMETVILTCNPETPDASYLWWMNGQSLPMTHRMQLSETNRTLFLFGVTKYTAGPYECEIWNSGSASRSDPVTLNLLHGPDLPRIFPSVTSYYSGENLDLSCFADSNPPAQYSWTINGKFQLSGQKLFIPQITPKHNGLYACSARNSATGEESSTSLTIRVIAPPGLGTFAFNNPT Predict reactive species |
| Full Name | pregnancy specific beta-1-glycoprotein 11 |
| Calculated Molecular Weight | 219 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC020711 |
| Gene Symbol | PSG11 |
| Gene ID (NCBI) | 5680 |
| RRID | AB_2171065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSG11, also named as PSG13, PSG14, PSBG-11 and PSBG-13, belongs to the immunoglobulin superfamily and CEA family. PSBG are produced in high quantity during pregnancy. PSG11 and other PSGs induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. (PMID:11327547)
Publications
What published studies show
The PSG11 Polyclonal antibody (Cat# 16352-1-AP) has a total of 1 citation. It appears in Frontiers in Genetics (2021), where it was used in Western Blot to study telomeric double-strand breaks and TERRA recruitment in human cells. The associated research field is telomere biology and DNA repair mechanisms.
| Species | Application | Title |
|---|---|---|
Front Genet Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA. |
