PSG8 Recombinant monoclonal antibody

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• PSG8 Recombinant monoclonal antibody (87878-3-RR)

• 

  WB analysis using 87878-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87878-3-RR (PSG8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 87878-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 87878-3-RR (PSG8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 87878-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 87878-3-RR (PSG8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 87878-3-RR

  Biolayer interferometry (BLI) kinetic assays of 87878-3-RR against Human PSG8 were performed. The affinity constant is 0.636 nM.

PSG8 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87878-3-RR

Clone No.260018G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Pregnancy-specific beta-1-glycoprotein 8, Pregnancy-specific glycoprotein 8, PS-beta-G-8, PSBG-8

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87878-3-RR targets PSG8 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg8783

  Product name: Recombinant human PSG8 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 35-426 aa of NM_182707.3

  Sequence: QVTIEAQPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQIRDLYHYITSYVVDGQIIIYGPAYSGRETIYSNASLLIQNVTQEDAGSYTLHIIMGGDENRGVTGHFTFTLYLETPKPSISSSKLNPREAMEAVSLTCDPETPDASYLWWMNGQSLPMSHRLQLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPFTLNLLPKLPKPYITINNLKPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDQYGGIRSYPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINGKFQLSGQKLFIPQITTKHSGLYACSVRNSATGKESSKSMTVKVSGKRIPVSLAIGI

• Full Name: pregnancy specific beta-1-glycoprotein 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-75 kDa
• GenBank Accession Number: NM_182707.3
• Gene Symbol: PSG8
• Gene ID (NCBI): 440533
• RRID: AB_3745803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UQ74-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).