PSIP1 Monoclonal antibody

PSIP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9E7

Cat no : 67723-1-Ig

Synonyms

CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2

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Validation Data Gallery

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  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67723-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67723-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67723-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells, U2OS cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67723-1-Ig targets PSIP1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSIP1 fusion protein Ag30562
• Full Name: PC4 and SFRS1 interacting protein 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_021144
• Gene Symbol: PSIP1
• Gene ID (NCBI): 11168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSIP1 enables DNA-binding transcription factor binding activity, chromatin binding activity and transcription coactivator activity. It is involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II.