PSIP1 Recombinant antibody, PBS Only

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  WB analysis using 85395-1-RR (same clone as 85395-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85395-1-RR (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 85395-1-RR (same clone as 85395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85395-1-RR (same clone as 85395-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (85395-1-RR, Clone: 242841C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85395-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85395-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85395-1-RR against Human PSIP1 were performed. The affinity constant is below 1 pM.

PSIP1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85395-1-PBS

Clone No.242841C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

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Product Information

85395-1-PBS targets PSIP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSIP1 fusion protein Ag21886
• Full Name: PC4 and SFRS1 interacting protein 1
• Calculated Molecular Weight: 530 aa, 60 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC064135
• Gene Symbol: PSIP1
• Gene ID (NCBI): 11168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75475
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.