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  • Western Blot (WB) analysis of various lysates using PSIP1 Recombinant antibody (85395-1-RR)

    WB analysis using 85395-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85395-1-RR (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSIP1 Recombinant antibody (85395-1-RR)

    IHC staining of human liver cancer using 85395-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSIP1 Recombinant antibody (85395-1-RR)

    IHC staining of human liver cancer using 85395-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSIP1 Recombinant antibody (85395-1-RR)

    IHC staining of human colon cancer using 85395-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSIP1 Recombinant antibody (85395-1-RR)

    IHC staining of human colon cancer using 85395-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85395-1-RR (PSIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSIP1 Recombinant antibody (85395-1-RR)

    IF Staining of HepG2 using 85395-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (85395-1-RR, Clone: 242841C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85395-1-RR

    Affinity and Kinetic Characterization of 85395-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85395-1-RR against Human PSIP1 were performed. The affinity constant is below 1 pM.

PSIP1 Recombinant antibody

PSIP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85395-1-RR
Clone No.242841C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242841C5, CLL associated antigen KW 7, CLL-associated antigen KW-7, DFS70, LEDGF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells. Daudi cells, HEK-293T cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85395-1-RR targets PSIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSIP1 fusion protein Ag21886 Predict reactive species
Full Name PC4 and SFRS1 interacting protein 1
Calculated Molecular Weight 530 aa, 60 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC064135
Gene Symbol PSIP1
Gene ID (NCBI) 11168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75475
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.

Protocols

Product Specific Protocols
WB protocol for PSIP1 antibody 85395-1-RRDownload protocol
IHC protocol for PSIP1 antibody 85395-1-RRDownload protocol
IF protocol for PSIP1 antibody 85395-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols