Anti-Human PSMA/GCPII Rabbit Recombinant Antibody

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  FC experiment of LNCaP using 98279-1-RR

  1x10^6 LNCaP cells (left) or HeLa (right) were surface stained with 0.25 ug Anti-Human FOLH1 Rabbit RecAb (98279-1-RR, Clone: 242026E6) (red),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue)and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98279-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98279-1-RR against Human PSMA/GCPII were performed. The affinity constant is 49.6 nM.

PSMA/GCPII Uni-rAb^® Recombinant Antibody for FC

Cat No. 98279-1-RR

Clone No.242026E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

FOLH1, PSMA, 242026E6, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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Tested Applications

• Positive FC detected in: LNCaP cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98279-1-RR targets PSMA/GCPII in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: folate hydrolase (prostate-specific membrane antigen) 1
• Calculated Molecular Weight: 84KD
• GenBank Accession Number: NM_004476.3
• Gene Symbol: PSMA
• Gene ID (NCBI): 2346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q04609
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

PSMA (Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.