PSMA4 Polyclonal antibody

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• PSMA4 Polyclonal antibody (11943-2-AP)

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• KD/KO Validated

PSMA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11943-2-AP

Publications(6)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HC9, Proteasome subunit alpha type-4, PSC9

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  WB analysis using 11943-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 11943-2-AP

  WB result of PSMA4 antibody (11943-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA4 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 11943-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse spleen using 11943-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of BxPC-3 using 11943-2-AP

  IP result of anti-PSMA4 (IP:11943-2-AP, 4ug; Detection:11943-2-AP 1:500) with BxPC-3 cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11943-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11943-2-AP (PSMA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse kidney tissue, mouse spleen tissue, HeLa cells, K-562 cells, U-937 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: human pancreas cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11943-2-AP targets PSMA4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2542

  Product name: Recombinant human PSMA4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-237 aa of BC056249

  Sequence: MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 4
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC056249
• Gene Symbol: PSMA4
• Gene ID (NCBI): 5685
• RRID: AB_874213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25789
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA4 (Proteasome Subunit Alpha 4) is a gene that encodes the alpha-4 subunit of the 20S proteasome core particle, which forms part of the outer alpha-ring structure of the barrel-shaped 20S complex. As an integral component of the 26S proteasome, PSMA4 participates in ATP- and ubiquitin-dependent protein degradation, a process essential for the regulation of cell cycle progression, signal transduction, and protein quality control. In addition, the 20S core particle can associate with regulatory complexes such as PA28 or PA200 to mediate ubiquitin-independent protein degradation, which plays important roles in pathways including antigen peptide generation and spermatogenesis. Dysregulation of PSMA4 expression or function has been implicated in various pathological conditions, including cancer and neurodegenerative diseases, highlighting its critical role in maintaining cellular proteostasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	J Biol Chem; PITHD1 is a proteasome-interacting protein essential for male fertilization.; Authors - Hiroyuki Kondo
mouse	IHC	Sci Rep; Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.; Authors - Monica Benvenuto
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
human	WB	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang
human	WB	Cell Death Dis; Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib; Authors - Yu Feng
human	IHC	Cell Death Discov; A comparative analysis of serum and tissue proteomic profiles in non-small cell lung cancer patients with or without brain metastasis.; Authors - Yongtao Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Aleksandr (Verified Customer) (07-29-2025)

Perfect antibody! Detection in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

PSMA4 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:11943-2-AP)

Tsimafei (Verified Customer) (08-12-2024)

Excellent antibody. The membrane was incubated with primary antibody at 4C, ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

PSMA4 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11943-2-AP)