Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68203 targets PSMA4 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32626 Product name: Recombinant human PSMA4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-237 aa of BC056249 Sequence: MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK Predict reactive species |
| Full Name | proteasome (prosome, macropain) subunit, alpha type, 4 |
| Calculated Molecular Weight | 237 aa, 27 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC056249 |
| Gene Symbol | PSMA4 |
| Gene ID (NCBI) | 5685 |
| RRID | AB_3084439 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25789 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMA4 (Proteasome Subunit Alpha 4) is a gene that encodes the alpha-4 subunit of the 20S proteasome core particle, which forms part of the outer alpha-ring structure of the barrel-shaped 20S complex. As an integral component of the 26S proteasome, PSMA4 participates in ATP- and ubiquitin-dependent protein degradation, a process essential for the regulation of cell cycle progression, signal transduction, and protein quality control. In addition, the 20S core particle can associate with regulatory complexes such as PA28 or PA200 to mediate ubiquitin-independent protein degradation, which plays important roles in pathways including antigen peptide generation and spermatogenesis. Dysregulation of PSMA4 expression or function has been implicated in various pathological conditions, including cancer and neurodegenerative diseases, highlighting its critical role in maintaining cellular proteostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PSMA4 antibody CL488-68203 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
