PSMA5 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• PSMA5 Polyclonal antibody, PBS Only (31384-1-PBS)

• 

  WB analysis using 31384-1-AP (same clone as 31384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31384-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 31384-1-AP (same clone as 31384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31384-1-AP (same clone as 31384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 31384-1-AP (same clone as 31384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (31384-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31384-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 31384-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Proteasome subunit alpha type-5, PSC5, ZETA

---

Product Information

31384-1-PBS targets PSMA5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34928

  Product name: Recombinant human PSMA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-241 aa of BC103751

  Sequence: MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 5
• Calculated Molecular Weight: 241 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC103751
• Gene Symbol: PSMA5
• Gene ID (NCBI): 5686
• RRID: AB_3669959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P28066
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)