PSMA5 Recombinant antibody

• 

  IF Staining of HeLa using 84424-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 84424-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 84424-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

PSMA5 Recombinant Antibody for IF/ICC, ELISA

Cat No. 84424-4-RR

Clone No.241629H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

ZETA, PSC5, Proteasome subunit alpha type-5, 241629H2

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells, U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84424-4-RR targets PSMA5 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSMA5 fusion protein Ag34928
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 5
• Calculated Molecular Weight: 241 aa, 26 kDa
• GenBank Accession Number: BC103751
• Gene Symbol: PSMA5
• Gene ID (NCBI): 5686
• RRID: AB_3671951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)