CoraLite® Plus 488-conjugated PSMA7 Monoclonal antibody

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  WB analysis using CL488-67817

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67817 (PSMA7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using CL488-67817

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PSMA7 antibody (CL488-67817, Clone: 1F2C6 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

PSMA7 Monoclonal Antibody for WB, IF/ICC

Cat No. CL488-67817

Clone No.1F2C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC

Synonyms

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67817 targets PSMA7 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7821

  Product name: Recombinant human PSMA7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-248 aa of BC004427

  Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC004427
• Gene Symbol: PSMA7
• Gene ID (NCBI): 5688
• RRID: AB_3672964
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14818
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named as HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis.(PMID: 19442227).