PSMA8 Monoclonal antibody

• Featured Product
• KD/KO Validated

PSMA8 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G2G3

Cat no : 68123-1-Ig

Synonyms

PSMA7L, PSMA8

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Validation Data Gallery

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  WB analysis using 68123-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 68123-1-Ig

  rat lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 68123-1-Ig

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 68123-1-Ig (PSMA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68123-1-Ig

  WB result of PSMA8 antibody (68123-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

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  IHC staining of human colon cancer using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68123-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68123-1-Ig (PSMA8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68123-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMA8 antibody (68123-1-Ig, Clone: 2G2G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, rat lung tissue, mouse lung tissue, HeLa cells, HuH-7 cells, NCI-H1299 cells, A549 cells, pig lung tissue, rabbit lung tissue
• Positive IHC detected in: mouse testis tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue, rat liver tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68123-1-Ig targets PSMA8 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA8 fusion protein Ag5138
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 8
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC042820
• Gene Symbol: PSMA8
• Gene ID (NCBI): 143471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

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