PSMB11 Polyclonal antibody, PBS Only

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• PSMB11 Polyclonal antibody, PBS Only (55143-1-PBS)

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  WB analysis of Jurkat using 55143-1-AP (same clone as 55143-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55143-1-AP (PSMB11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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  IHC staining of human thymus using 55143-1-AP (same clone as 55143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55143-1-AP (PSMB11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 55143-1-AP (same clone as 55143-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PSMB11 (55143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55143-1-PBS in a different storage buffer formulation.

PSMB11 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 55143-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

55143-1-PBS targets PSMB11 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: proteasome (prosome, macropain) subunit, beta type, 11
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: NM_001099780
• Gene Symbol: PSMB11
• Gene ID (NCBI): 122706
• RRID: AB_10863288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A5LHX3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.